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New products for exosome isolation to mRNA purification
June 2015
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Hitachi Chemical Diagnostics
 
The ExoComplete system isolates exosomal mRNA from biological samples such as plasma in a 96-well filter plate format or large-volume samples such as urine in a single collection tube
format. Exosomes and microvesicles (EMVs) in body fluids are captured with Hitachi Chemical’s Exosome Isolation Plate or Exosome Collection Tube with a proprietary filter material. The highly porous material allows fast filtration of biological samples without clogging and reproducible isolation of EMVs without the use of conventional ultracentrifugation. After lysing the captured EMVs, mRNA can be isolated through hybridization with single-strand oligo (dT) immobilized in the wells of the 96-well mRNA Capture Plate. A wide variety of downstream applications become available following isolation of mRNA, including mRNA quantification, single- and double-stranded cDNA synthesis, RT-PCR, real time quantitative RT-PCR (qPCR), and next- generation RNA sequencing.
 
Hitachi Chemical Diagnostics

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