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High-fidelity blunt-end PFU-50 polymerase
July 2017
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Empirical Bioscience
 
Empirical is now offering PFU-50, an ultra-high-fidelity polymerase for PCR applications. In addition to delivering a fidelity that is 50 times greater than Taq polymerases and a rate of accuracy that is twofold higher than standard Pfu polymerases, PFU-50 also offers improved processing times, shorter elongation times and better ligation.
 
The genetically engineered enzyme invokes the polymerization of nucleotides into duplex DNA in 5’→3’ direction but does not possess 5’→3’ exonuclease replacement activity. Instead, it offers a 3’→5’ exonuclease proofreading capability, significantly increasing the fidelity of DNA compared to Taq polymerase. These characteristics lend themselves well to applications like cloning and next-generation sequencing where accuracy, simple ligation and speed are key.
 
Empirical Bioscience

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